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The specificity of PCR-based protocols for detection of Erwinia amylovora

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Publication Type  Journal Article
Year of Publication  2011
Authors  Powney, R.; Beer, S.V.; Plummer, K.; Luck, J.; Rodoni, B.
Journal Title  Australasian Plant Pathology
Volume  40
Issue  1
Pages  87-97
Abstract  

An evaluation of seven published conventional PCR protocols used for the detection of Erwinia amylovora has shown that six out of the seven protocols tested were not specific for all strains of E. amylovora. A collection of 40 genetically diverse strains of E. amylovora and 55 geographically diverse bacteria that are closely related or share the same ecological niche as E. amylovora were used to test the seven PCR protocols. All bacteria were tested for virulence by inoculation of immature pear fruit and for cultural characteristics on selective media. Only one PCR protocol, Taylor et al. (2001), was specific for all strains of E. amylovora and was able to differentiate E. amylovora from all other bacteria tested. Diagnostic laboratories may need to review their testing procedures in light of these findings.

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The specificity of PCR-based protocols for detection of Erwinia amylovora

 

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