You are here: Home > Publications/research > Accumulating Variation at Conserved Sites in Potyvirus Genomes is Driven by Species Discovery and Affects Degenerate Primer Design

Accumulating Variation at Conserved Sites in Potyvirus Genomes is Driven by Species Discovery and Affects Degenerate Primer Design

Publication Type  Journal Article
Year of Publication  2008
Authors  Zheng, L.; Wayper, P.; Gibbs, A.; Fourment, M.; Rodoni, B.; Gibbs., M.
Journal Title  PLoS ONE
Abstract  

Unknown and foreign viruses can be detected using degenerate primers targeted at conserved sites in the known viral gene sequences. Conserved sites are found by comparing sequences and so the usefulness of a set of primers depends crucially on how well the known sequences represent the target group including unknown sequences.

URL  http://www.plosone.org/article/info:doi/10.1371/journal.pone.0001586
Export  Tagged XML BibTex